Application of a 235 rRNA Fluorescent In Situ Hybridization method for rapid detection of Salmonella sp. in slaughtered pigs

摘要

Pork products contamination during the slaughter process represents an important vehicle of Salmonella sp. dissemination to humans. It\u27s urgent to develop rapid, sensitive and accurate methods that allow the detection of a large number of Sa/monel/a-positive samples, in order to control these risks efficiently and in a practical time. This study evaluates the suitability of Fluorescent In Situ Hybridization (FISH) method as a rapid screening tool for Salmonella sp. deteclton in pork carcasses, as well in some risk tissues (ileum, ileocolic and mandibular lymph nodes and tonsils), which could be involved in Salmonella contamination during slaughter process. For that, FISH was comparatively analysed with the labour intenstve reference microbiological culture method (ISO 6579:2002) whose results were previously published by the same authors.